Oligo Synthesis

Custom DNA Oligo & Diagnostic Probe Synthesis Services

Reliable oligonucleotide synthesis is fundamental to the success of modern molecular biology research and diagnostic development. We provide high-quality Custom DNA Oligo and Diagnostic Probe synthesis services tailored to the needs of research laboratories, academic institutions, hospitals, biotechnology companies, and diagnostic kit developers. By combining advanced synthesis technologies with rigorous quality control processes, we ensure exceptional sequence accuracy, high purity, and consistent performance across every batch.

Our Custom DNA Oligo services support a wide range of molecular biology applications, including PCR and RT-PCR primers, DNA sequencing primers, cloning and mutagenesis oligos, CRISPR guide oligos, NGS adapters and indexes, gene assembly, and synthetic biology workflows. Oligonucleotides can be synthesized according to customer specifications, with flexible options for sequence length, synthesis scale, purification methods, and specialized modifications to meet the requirements of both routine research and advanced genomic applications.

In addition to standard oligonucleotide synthesis, we offer comprehensive Diagnostic Probe and Modified Oligo solutions for molecular diagnostics and assay development. Our capabilities include the synthesis of TaqMan® probes, Dual-Quencher probes, Molecular Beacons, SNP Genotyping probes, FISH probes, RPA/RAA probes, and other custom-designed detection oligonucleotides. A broad range of fluorescent dyes and quenchers are available, including FAM, HEX, VIC, Cy3, Cy5, ROX, TAMRA, BHQ1, BHQ2, and many other modifications commonly used in qPCR, multiplex assays, pathogen detection, and genomic analysis.

Modifications

TAMRA

5’FAM-3’TAMRA, 5’HEX-3’TAMRA, 5’TET-3’TAMRA, 5’JOE-3’TAMRA

BHQ 1

5’FAM-3’BHQ1, 5’HEX-3’BHQ1, 5’VIC-3′BHQ1, 5’NED-3′BHQ1, 5’TAMRA-3′BHQ1, 5’JOE-3’BHQ1

BHQ 2

5’Cy5-3’BHQ2, 5’Cy3-3’BHQ2, 5’ROX-3’BHQ2

MGB

5’NED-3′ MGB, 5’TAMRA-3′ MGB, 5’ROX-3’MGB, 5’JOE-3’TAMRA, 5’Cy5-3’MGB, 5’Cy3-3’MGB, 5’VIC-3′MGB, 5’HEX-3’MGB, 5’FAM-3’MGB

DABCYL

5’FAM-3′ DABCYL, 5’HEX-3′DABCYL, 5’JOE-3′DABCYL, 5’ROX-3′DABCYL, 5’TAMRA-3′DABCYL, 5’CY5-3′DABCYL

Our products support numerous applications across life science research and diagnostics, including gene expression analysis, infectious disease detection, oncology research, genotyping, biomarker discovery, next-generation sequencing (NGS), and assay development. Whether you are conducting fundamental research, validating a molecular method, or developing a commercial diagnostic product, our custom oligonucleotide solutions are designed to deliver the sensitivity, specificity, and reproducibility your project demands.

Synthetic Specification

nmol

Turnaround Time

2 – 4 weeks

Standard Delivery Project

COA file, MS or HPLC report.

Purification

RP-HPLC/PAGE, etc

QC

RP-HPLC detection ≥90%, LC-MS detection molecular weight deviation <0.1%, Full wavelength scanning (optional)

Cross-Contamination

Conventional platform anti-pollution process

Delivery Form

Dry powder

We understand that every project presents unique challenges. Our technical team is available to assist with primer and probe design considerations, fluorophore and quencher selection, optimization strategies, and specification recommendations to help maximize assay performance. From early-stage research to commercial development, we are committed to providing dependable oligonucleotide synthesis services backed by responsive technical support and reliable turnaround times.

If you are looking for custom PCR primers, qPCR probes, sequencing primers, NGS oligos, FISH probes, or highly specialized modified oligonucleotides, contact us today to discuss your project requirements and receive a customized quotation.

HOW TO ORDER:

  1. Please log in to your account before placing an order.
  2. Download the Excel form provided below on this page.
  3. Fill out the form according to the instructions and guidelines provided within the file.
  4. Complete the online submission form available below.
  5. Upload the completed Excel form and submit the required information through the submission form.
  6. Our team will review your submission and contact you for further processing or clarification if needed.

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