In vitro cell culture, in order to preserve the biological activity of cells for a long time, the cells must be cryopreserved,and resuscitated when necessary.At present, the most commonly used method for cell cryopreservation is liquid nitrogen method, which mainly uses the addition of an appropriate amount of protective agent to slowly cool the cells to a specified temperature range, so as to achieve the purpose of protecting cells.
The Primary Cell Cryopreservation Solution (SFC1) Medium is a special cryopreservation product developed by Procell for primary cells, mainly for the cryopreservation of mesenchymal stem cells, smooth muscle cells, fibroblasts and chondrocytes derived from a variety of tissue species. The product formula contains a self-developed cryoprotectant and a dual cryoprotectant of 10% DMSO. The product formula is clear , does not contain serum or animal-derived proteins, which can reduce contamination risk from bacteria, viruses, and mycoplasma, ensuring the safety of frozen cells.
Compared with traditional cryopreservation media, there is no need for cumbersome and time-consuming procedural cryopreservation steps or expensive procedural coolers, and the cells can be directly resuspended and placed in an ultra-low temperature freezer at -80℃ to ensure that the ultra-low temperature temperature is stable and frozen for 1 year. If you want to store liquid nitrogen for a medium and long term, you need to put it in a -80°C ultra-low temperature freezer for 24 h before moving it to a liquid nitrogen tank for long-term storage.