• Generate circular ssDNA templates for rolling circle replication or rolling circle
• transcription experiments
• Generate ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays
• Used for cfDNA/ctDNA sequencing, methylation sequencing, etc.
Circle Ligase is a thermostable ATP-dependent ligase that can catalyze the self-circularization of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) strands simultaneously possessing a 5′-phosphate group and a 3′-hydroxyl group in the absence of complementary sequences, with the length of single-stranded DNA (ssDNA)/single-stranded RNA (ssRNA) being greater than 15bp.
