Advanced RPMI-1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI-1640, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced RPMI-1640, with no adaptation, include Vero, Raji, Daudi, Jurkat, Clone E6-1, THP-1, 4T1, CT26.WT, PC-12 (High differentiation), 22RV1, BxPC-3, B16-F10, etc.
Advanced RPMI-1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI-1640, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced RPMI-1640, with no adaptation, include Vero, Raji, Daudi, Jurkat, Clone E6-1, THP-1, 4T1, CT26.WT, PC-12 (High differentiation), 22RV1, BxPC-3, B16-F10, etc.
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