ABScript II One Step SYBR Green RT-qPCR Kit 250RXN

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Product Features
  • Reverse transcription and qPCR are operated in the same reaction tube, which simplifies the experimental operation and reduces the risk of contamination.
  • The results are reliable: the recombinant reverse transcriptase reduced the activity of RNase H, improved the thermal stability and cDNA synthesis efficiency, and the hot-started Taq DNA Polymerase increased the specificity of PCR amplification.
  • High sensitivity: the optimized Buffer system greatly improved the amplification efficiency of PCR reaction, and the detection sensitivity was up to 0.1pg total RNA.
Product Applications
real-time PCR
SKU: RK20404-250rxn Categories: , , , Brand:

ABScript II One Step SYBR Green RT-qPCR Kit is a special kit for one-step RT-qPCR reaction by using SYBR Green I chimeric fluorescence. The kit takes RNA as the template, uses gene-specific primers, and reverse transcription and PCR reaction can be carried out continuously in the same tube without additional pipe opening and pipetting operations, greatly improving the detection flux and effectively preventing contamination. This reaction system can detect the amplification products in real time, greatly improving the detection sensitivity, and omitting the electrophoresis step after PCR reaction, which is very suitable for the detection of RNA virus and other trace RNA. This product integrates the superiority of ABScript II Reverse Transcriptase and Taq DNA Polymerase, cooperate with optimized buffer system, with high amplification efficiency and high amplification specificity, one-step RT – qPCR reaction can be stable. In addition, all the enzymes used in the reaction are made into enzyme Mix, which is easier and more convenient to operate.

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