Product Features • This enzyme does not require ATP as a cofactor，but requires a pre-adenylated
• substrate.
• It can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of • DNA.
• More than 90% enzyme activity was maintained when the salt concentration reached
• 500 mM.
• Strict batch performance and stability quality control.
Product Applications • This enzyme can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´
• end of DNA.
• Ligation of 5 ‘preadenylation adaptor to DNA fragments for NGS library construction.
5’App-T4 DNA Ligase is a mutant of T4 DNA Ligase without adenylation function, which can specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of DNA. This enzyme does not require ATP as a cofactor for ligation, but requires a pre-adenylated substrate. This enzyme reduces background ligation (chimera formation) during NGS library construction, because it can only use 5 ‘preadenylation adapter to the DNA fragments.
