0.5% EDTA Solution (100 mL)

EDTA is a chemical chelating agent that can chelate divalent metal ions such as Ca²⁺ and Mg²⁺. Its solution is also called Versene solution. Because Ca²⁺ and Mg²⁺ are important components of cell membranes and have the function of agglutinating cells, EDTA can destroy the connection between cells and promote the dissociation of cells into single cells. EDTA is less toxic, cheap, convenient to use, and does not damage cell surface molecules. It only binds to Ca²⁺ and Mg²⁺, so it is suitable for cell digestion where cell surface molecules need to be detected. It is also often mixed with trypsin solution in a certain proportion.