ABScript miRNA First-Strand Synthesis Kit (by tailing A) 20RXN

This kit is suitable for cDNA first strand synthesis using microRNA as template through the tail addition method, where the Poly (A) tail addition reaction and reverse transcription reaction at the 3 ‘end of miRNA can be efficiently carried out simultaneously. ABScript miRNA-A Enzyme Mix contains Poly (A) Polymerase (PAP) and reverse transcriptase. PAP is mainly used to add Poly (A) tails at the 3 ‘end of RNA molecules, and can also specifically recognize single stranded RNA, effectively avoiding RT reactions of pre-miRNA with double stranded or stem-loop structures. The modified reverse transcriptase lacks of RNase H activity and increases its affinity with RNA, resulting in a significant improvement in the efficiency and sensitivity of miRNA reverse transcription. The obtained cDNA can be directly used for qPCR detection using either SYBR Green dye-base or Taqman probe-base reagent.