Grace’s medium for insect Cells (500 mL)

Grace’s Insect Medium is Grace’s modified Wyatt Medium, which is more similar in chemical composition to insect hemolymph, and Grace used this medium to successfully establish the first insect continuous cell line. Grace’s Insect Medium was originally used for the culture of Antherea eucalypti cells of the Australian White Star Orange-Orange Silkworm Moth, and with appropriate supplementation, Grace’s Insect Medium can be used to grow a variety of insect cells, including cells of a variety of lepidoptera and some dipteroptera.