Advanced DMEM (500 mL)

Advanced DMEM is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced DMEM, with no adaptation, include Vero, COS-7, A549, 3T3-L1, HuH-7, MDA-MB-231, SW620, NRK-52E, A-673, C2C12, RH-35, Ishikawa, CHL, etc.
Advanced DMEM medium can achieve the serum-reduced effect due to the addition of the following ingredients: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin and various trace elements (sodium selenite, ammonium metavanadate, copper sulfate and manganese chloride).