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Advanced DMEM/F12 (500 mL)
Login/Register to see pricesIngredient Statement
With
• 3151 mg/L D-Glucose
• 365 mg/L L-Glutamine
• 110 mg/L Sodium Pyruvate
• 8.1 mg/L Phenol red
• NEAA
• Purified Water Dissolvent
Without
• HEPES
Advanced DMEM/F12 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic DMEM/F12, serum supplementation can be reduced by 50–90% with no change in cellular prolife ratio or morphology. Many cell lines do not need to be domesticated to use this medium. Cells successfully cultured in Advanced DMEM/F12, with no adaptation, include Vero, LX-2, KGN, C8-D1A, Hela, 4T1, NIH-3T3, KLE, ARPE-19, JHH-7, NCI-H2009, etc.
Advanced DMEM/F12 medium can achieve the serum-reduced effect due to the addition of the following ingredients: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, bovine serum albumin and various trace elements (sodium selenite, ammonium metavanadate, copper sulfate and manganese chloride).
SYSTEM SERTIFICATION
SPECIFICATION
| Concentration | 1× |
| Volume | 500mL |
| Form | Liquid |
| Bacteria Detection | Negative |
| Fungi Detection | Negative |
| Mycoplasma Detection | Negative |
| Endotoxin Content | < 3 EU/mL |
| pH | 7.0-7.4 |
| Animal Origin Ingredient | Without |
| Green Features | Sustainable packaging |
| Valid Period | 12 months |
| Storage Condition | This product can be stored at 2-8°C for 12 months with shading light. |
| Shipping Condition | Ice bag |
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